| 1 | Amanda Nauman (USA) SDG - Muscle Monster | 0:44:46 |
| 2 | Jenna Blandford (USA) Women's Project Pedal p/b VO2 | 0:00:28 |
| 3 | Laura Winberry (USA) Speedvagen Family Racing | 0:00:54 |
| 4 | Jennifer Malik (USA) Women's CX Project | 0:01:10 |
| 5 | Megan Barr (USA) Angry Catfish | 0:01:23 |
| 6 | Rebecca Blatt (USA) Van Dessel Factory Team | 0:01:35 |
| 7 | Corey Coogan Cisek (USA) Power Fix CX | 0:01:49 |
| 8 | Nicole Dorinzi (USA) PHI Hotel Group / P-K Express p/b Holiday Inn Express | 0:01:52 |
| 9 | Sydney Guagliardo (USA) PSIMET Racing | 0:01:57 |
| 10 | Maria Larkin (USA) Chicago Cuttin Crew | 0:02:15 |
| 11 | Meghan Korol (USA) Team Handmade | 0:02:39 |
| 12 | Abby Watson (USA) The Athletic | 0:04:02 |
| 13 | Amanda Schaap (USA) Speed Merchants | 0:06:13 |
| 14 | Clio Dinan (USA) KUDU CX Collective | 0:07:14 |
| DNS | Chelsea Weidinger (USA) Mash SF | Row 14 - Cell 2 |
| DNS | Kennedy Adams (USA) Women's CX Project | Row 15 - Cell 2 |
| DNS | Lindsay Knight (USA) Tenspeed Hero | Row 16 - Cell 2 |
