| 1 | Helen Wyman (GBr) Kona Factory Racing | 0:38:49 |
| 2 | Laura Van Gilder (USA) Mellow Mushroom | 0:00:48 |
| 3 | Arley Kemmerer (USA) C3-Twenty20 Cycling | 0:01:21 |
| 4 | Maureen Bruno Roy (USA) Bob's Red Mill p/b Seven Cycles | 0:01:25 |
| 5 | Nicole Thiemann (USA) Team CF | 0:01:44 |
| 6 | Serena Gordon (USA) All Access Racing | 0:02:42 |
| 7 | Katrina Dowidchuk (USA) Colavita-EspnW NJ | 0:02:53 |
| 8 | Stacey Barbossa (USA) NJ/NY Colavita Women | 0:02:55 |
| 9 | Ally Stacher (USA) Specialized-lululemon | 0:03:20 |
| 10 | Julie Hunter (USA) BikeBeat-VaAssetGroup | 0:03:24 |
| 11 | Carolyn Popovic (USA) Team CF | 0:03:32 |
| 12 | Brittlee Bowman (USA) House Industries-Richard Sachs-RGM | 0:03:33 |
| 13 | Avanell Schmitz (USA) BikeBeat-VaAssetGroup | 0:03:35 |
| 14 | Allyson Tufano (USA) Sportif Coaching Group-Patapsco Bike | Row 13 - Cell 2 |
| 15 | Katherine Shields (USA) Mock Orange Pro CX Team | 0:03:38 |
| 16 | Jessica Kutz (USA) Team EPS-CSS p/b Shebell & Shebell | 0:03:45 |
| 17 | Lauren Dagostino (USA) NJ/NY Colavita Women | 0:03:52 |
| 18 | Frances Morrison (USA) C3-Twenty20 Cycling | 0:04:23 |
| 19 | Traci Rodosta (USA) Breakaway Velo | 0:04:35 |
| 20 | Erica Yozell (USA) Bicycle Therapy-Melitta | 0:04:42 |
| 21 | Victoria Gates (USA) JAM Fund-NCC | 0:04:50 |
| 22 | Jennifer Tillman (USA) C3-Twenty20 Cycling | 0:05:06 |
| 23 | Kathleen Wulfkuhle (USA) C3-Twenty20 Cycling | 0:05:13 |
| 24 | Jocelyn Mauldin (USA) NEBC p/b CycleLoft | 0:05:21 |
| 25 | Linda Molendyke (USA) Bicycle Therapy-Melitta | 0:05:25 |
| 26 | Bailey Semian (USA) C3-Twenty20 Cycling | 0:05:27 |
| 27 | Kristine Church (USA) Peanut Butter & Co. Human Zoom | 0:05:37 |
| 28 | Kaitlyn Lawrence (USA) C3-Twenty20 Cycling | 0:05:49 |
| 29 | Ginny Jeppi (USA) Sublime Athletics Velo | 0:05:59 |
| 30 | Dana McAllister (USA) Peanut Butter & Co. Human Zoom | 0:06:10 |
| 31 | Erin Mascelli (USA) Peanut Butter & Co. Human Zoom | 0:06:18 |
| 32 | Stephanie Thompson (USA) WD Racing -NYC Velo | Row 31 - Cell 2 |
| 33 | Lisa Most (USA) Wissahickon-Engin Cycles | Row 32 - Cell 2 |
| 34 | Caitlin Thompson (USA) Peanut Butter & Co. Human Zoom | Row 33 - Cell 2 |
| 35 | Jennifer Pope (USA) Rogue Velo Racing | Row 34 - Cell 2 |
| 36 | Heidi Wood (USA) Alki-Rubicon | Row 35 - Cell 2 |
| 37 | Emily Shields (USA) Mock Orange Pro CX Team | Row 36 - Cell 2 |
| 38 | Lauren Festa (USA) Peanut Butter & Co. Human Zoom | Row 37 - Cell 2 |
| 39 | Abigail Isolda (USA) NYCross.com p/b VOmax | Row 38 - Cell 2 |
