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| 1 | Helen Wyman (GBr) Kona Factory Racing | 0:39:32 |
| 2 | Arley Kemmerer (USA) C3-Twenty20 Cycling | 0:01:19 |
| 3 | Nicole Thiemann (USA) Team CF | 0:01:27 |
| 4 | Julie Hunter (USA) BikeBeat-VaAssetGroup | 0:01:41 |
| 5 | Maureen Bruno Roy (USA) Bob's Red Mill p/b Seven Cycles | 0:01:48 |
| 6 | Serena Gordon (USA) All Access Racing | 0:02:00 |
| 7 | Brittlee Bowman (USA) House Industries-Richard Sachs-RGM | 0:02:12 |
| 8 | Stacey Barbossa (USA) NJ/NY Colavita Women | 0:02:35 |
| 9 | Allyson Tufano (USA) Sportif Coaching Group-Patapsco Bike | 0:02:53 |
| 10 | Katrina Dowidchuk (USA) Colavita-EspnW NJ | Row 9 - Cell 2 |
| 11 | Emily Shields (USA) Mock Orange Pro CX Team | 0:03:07 |
| 12 | Ally Stacher (USA) Specialized-lululemon | 0:03:15 |
| 13 | Katherine Shields (USA) Mock Orange Pro CX Team | 0:03:23 |
| 14 | Lauren Dagostino (USA) NJ/NY Colavita Women | 0:03:28 |
| 15 | Carolyn Popovic (USA) Team CF | 0:03:29 |
| 16 | Cheryl Sornson (USA) Team CF | 0:03:35 |
| 17 | Avanell Schmitz (USA) BikeBeat-VaAssetGroup | 0:03:51 |
| 18 | Kathleen Wulfkuhle (USA) C3-Twenty20 Cycling | 0:03:54 |
| 19 | Jessica Kutz (USA) Team EPS-CSS p/b Shebell & Shebell | 0:04:19 |
| 20 | Erica Yozell (USA) Bicycle Therapy-Melitta | 0:04:24 |
| 21 | Traci Rodosta (USA) Breakaway Velo | 0:04:27 |
| 22 | Jennifer Tillman (USA) C3-Twenty20 Cycling | 0:04:56 |
| 23 | Victoria Gates (USA) JAM Fund-NCC | 0:05:02 |
| 24 | Kristine Church (USA) Peanut Butter & Co. Human Zoom | 0:05:38 |
| 25 | Amy Breyla (USA) C3-Twenty20 Cycling | 0:05:41 |
| 26 | Linda Molendyke (USA) Bicycle Therapy-Melitta | 0:05:50 |
| 27 | Jocelyn Mauldin (USA) NEBC p/b CycleLoft | 0:05:57 |
| 28 | Erin Mascelli (USA) Peanut Butter & Co. Human Zoom | 0:05:59 |
| 29 | Bailey Semian (USA) C3-Twenty20 Cycling | 0:06:19 |
| 30 | Dana McAllister (USA) Peanut Butter & Co. Human Zoom | 0:06:33 |
| 31 | Kaitlyn Lawrence (USA) C3-Twenty20 Cycling | 0:07:00 |
| 32 | Jennifer Pope (USA) Rogue Velo Racing | 0:07:02 |
| 33 | Heidi Wood (USA) Alki-Rubicon | 0:07:17 |
| 34 | Ginny Jeppi (USA) Sublime Athletics Velo | 0:07:28 |
| 35 | Lauren Festa (USA) Peanut Butter & Co. Human Zoom | 0:08:14 |
| 36 | Caitlin Thompson (USA) Peanut Butter & Co. Human Zoom | 0:09:03 |
| 37 | Stephanie Thompson (USA) WD Racing -NYC Velo | Row 36 - Cell 2 |
| 38 | Abigail Isolda (USA) NYCross.com p/b VOmax | Row 37 - Cell 2 |
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