| 1 | Melinda McCutcheon (USA) DNA-Cotton Sox | 0:50:46 |
| 2 | Amanda Miller (USA) Boulder Cycle Sport/YogaGlo | 0:00:18 |
| 3 | Sofia Gomez Villafane (Arg) TENSPEED HERO | 0:01:46 |
| 4 | Emily Kachorek (USA) Squid | 0:02:33 |
| 5 | Melissa Barker (USA) EVOL Elite Racing | 0:02:55 |
| 6 | Daniele Arman (USA) Tenspeed Hero | 0:03:13 |
| 7 | Caitlyn Vestal (USA) Feedback Sports Racing | 0:03:18 |
| 8 | Christa Ghent (USA) EVOL DevoElite Racing | 0:03:29 |
| 9 | Kristin Weber (USA) Boulder Cycle Sport/ YogaGlo | 0:03:52 |
| 10 | Hannah Williams (USA) Kent Eriksen Cycles Factory Team | 0:03:53 |
| 11 | Alexis Skarda (USA) Stan's Notubes Elite Women's Team | 0:04:35 |
| 12 | Amy Beisel (USA) Griggs Orthopedics | 0:04:50 |
| 13 | Clara Honsinger (USA) Team S+M | 0:04:55 |
| 14 | Lisa Hudson (USA) Feedback Sports | 0:05:21 |
| 15 | Suzie Livingston (USA) Fort Follies | 0:05:29 |
| 16 | Deidre Morrison (USA) Stages Cycling | 0:06:03 |
| 17 | Meghan Newlin (USA) SALT/ Full Cycle Boulder | 0:06:29 |
| 18 | Kristie Arend (USA) 50x15 | 0:06:51 |
| 19 | Tiziana Dehorney (USA) PDX/TI (Portland Titanium) | 0:07:26 |
| 20 | Ashley Zoerner (USA) Alpha Bicycle Company Vista Subaru | 0:08:24 |
| 21 | Rebecca Blatt (USA) Van Dessel | |
| 22 | Ksenia Lepikhina (USA) Cross Propz | |
| 23 | Abby Youngwerth (USA) Tenspeedhero | |
| 24 | Sarah Ginsbach (USA) Topo Designs Cycling Club | |
| 25 | Sarah Lukas (USA) Amy D Foundation | |
| DNF | Beth Ann Orton (USA) Team S+M Sellwood Cycle Repair | |
| DNF | Breeze Holicky (USA) EVOL DevoElite racing | |
| DNS | Meredith Miller (USA) Focus | |
