| 1 | Jen Luebke (Hagens Berman Supermint) | 1:00:21 |
| 2 | Skylar Schneider (Boels Dolmans Cycling Team) | 0:00:01 |
| 3 | Samantha Schneider (ISCorp Pro Cycling) | Row 2 - Cell 2 |
| 4 | Josie Talbot (ISCorp Pro Cycling) | Row 3 - Cell 2 |
| 5 | Valentina Scandolara (Roxsolt Attaquer) | Row 4 - Cell 2 |
| 6 | Shelby Reynolds (Monster Media Elite Women) | 0:00:02 |
| 7 | Lily Williams (Hagens Berman supermint) | Row 6 - Cell 2 |
| 8 | Christina Gokey Smith (Colavita Bialetti Pro Cycling T) | Row 7 - Cell 2 |
| 9 | Katie Clouse (DNA Cycling Team) | Row 8 - Cell 2 |
| 10 | Sharlotte Lucas (Roxsolt Attaquer) | Row 9 - Cell 2 |
| 11 | Tina Pic (Colavita Bialetti Pro Cycling T) | Row 10 - Cell 2 |
| 12 | Liza Rachetto (Hagens Berman supermint) | 0:00:03 |
| 13 | Carolyn Defoore (Wolfpack p b Hyperthreads) | Row 12 - Cell 2 |
| 14 | Peta Mullens (Hagens Berman Supermint) | Row 13 - Cell 2 |
| 15 | Mel Beale (LA Sweat) | Row 14 - Cell 2 |
| 16 | Rachel Langdon (Gray Goat Mobile Bullseye Total) | Row 15 - Cell 2 |
| 17 | Leighann Ganzar (Wolfpack p b Hyperthreads) | Row 16 - Cell 2 |
| 18 | Yussely Mendivil Soto (ISCorp Pro Cycling) | Row 17 - Cell 2 |
| 19 | Jessica Mundy (THE METEOR INTELLIGENTSIA) | 0:00:04 |
| 20 | Marta Morris (Stages Cycling) | Row 19 - Cell 2 |
| 21 | Anne Donley | 0:00:05 |
| 22 | Michaela Drummond (DNA Cycling Team) | Row 21 - Cell 2 |
| 23 | Daphne Karagianis (THE METEOR INTELLIGENTSIA) | Row 22 - Cell 2 |
| 24 | Jennifer Wagner (Wolfpack p b Hyperthreads) | Row 23 - Cell 2 |
| 25 | Marissa Axell (JL Velo Cycling Team) | Row 24 - Cell 2 |
| 26 | Faith Montreuil (Gray Goat Mobile Bullseye Total) | 0:00:06 |
| 27 | Ash Duban (THE METEOR INTELLIGENTSIA) | 0:00:09 |
| 28 | Nicole Pressprich (Point S Auto presented by Nokia) | Row 27 - Cell 2 |
| 29 | Rebecca Larson (Tulsa Wheelmen) | Row 28 - Cell 2 |
| 30 | Sheri Rothe (Wolfpack p b Hyperthreads) | Row 29 - Cell 2 |
| 31 | Rachel Plessing (ALP Cycles Racing) | 0:00:10 |
| 32 | Victoria Kanizer (CTH Performance Project) | 0:00:11 |
| 33 | Lisa Cordova (Cyclesport Specialized p b Musc) | Row 32 - Cell 2 |
| 34 | Ashley Weaver (Papa Johns Racing p b TREK) | 0:00:13 |
| 35 | Grace Chappell (Wolfpack p b Hyperthreads) | 0:00:14 |
| 36 | Whitney Allison (Hagens Berman Supermint) | 0:00:15 |
| 37 | Amity Gregg | Row 36 - Cell 2 |
| 38 | Sophie Russenberger (Point S Nokian) | Row 37 - Cell 2 |
| 39 | Aliesha Larsen (DNA Cycling Team) | Row 38 - Cell 2 |
| 40 | Ayesha Mcgowan | 0:00:22 |
| 41 | Hanna Muegge (DNA Cycling Team) | Row 40 - Cell 2 |
| 42 | Laurel Rathbun (Colavita Bialetti Pro Cycling T) | Row 41 - Cell 2 |
| 43 | Shelby Tramel (CycleSport Specialized p b Musc) | 0:00:23 |
| 44 | Allison Schroeder (Chicago Womens Elite Cycling) | Row 43 - Cell 2 |
| 45 | Maria Danker (Palmares Racing) | 0:00:25 |
| 46 | Christie Tracy (DNA Cycling Team) | 0:00:33 |
| 47 | Jennifer Sharp (Chicago Womens Elite Cycling) | Row 46 - Cell 2 |
| 48 | Caroline Baur (ISCorp Pro Cycling) | 0:00:50 |
| 49 | Samantha Verrill (Roxsolt Attaquer) | Row 48 - Cell 2 |
| 50 | Esther Meisels (Point S Nokian) | 0:00:54 |
| 51 | Lynn Wilson (Midwest Cycling Trek Stores Wom) | Row 50 - Cell 2 |
| 52 | Briana Clark (Gray Goat Mobile Bullseye Total) | 0:01:00 |
| 53 | Charlotte Culver (Roxsolt Attaquer) | 0:03:03 |
| 54 | Colleen Gulick (Pickle Juice Pro Cycling Team) | Row 53 - Cell 2 |
| 55 | Natalie Tapias (THE METEOR INTELLIGENTSIA) | 0:03:15 |
| 56 | Lauren Wissman (Chicago Womens Elite Cycling) | Row 55 - Cell 2 |
| 57 | Caro Poole (Leborne Womens Racing Fueled by) | Row 56 - Cell 2 |
| 58 | Ginger Boyd | Row 57 - Cell 2 |
| 59 | Emma Roberts (LA Sweat) | 0:03:21 |
| 60 | Alijah Beatty (Pickle Juice Pro Cycling team) | Row 59 - Cell 2 |
| 61 | Charlotte Backus (Prestige imports) | Row 60 - Cell 2 |
| 62 | Leah Thorvilson (Canyon SRAM Racing) | Row 61 - Cell 2 |
| 63 | Michelle Henry (Palmares Racing) | Row 62 - Cell 2 |
| 64 | Emily Jordan (Palmares Racing) | Row 63 - Cell 2 |
| 65 | Elizabeth Kieffer (Gray Goat Mobile Bullseye Total) | Row 64 - Cell 2 |
| 66 | Mia Cheeseman (Chicago Womens Elite Cycling) | Row 65 - Cell 2 |
| 67 | Katie Isermann (Chicago Womens Elite Cycling) | Row 66 - Cell 2 |
| DNF | Margaux Salas (Colavita Texas) | Row 67 - Cell 2 |
| DNF | Kimberly Pettit (Papa John;s Racing p b TREK) | Row 68 - Cell 2 |
| DNF | Maria Larkin (Chicago Womens Elite Cycling) | Row 69 - Cell 2 |
| DNF | Nicole Mertz (THE METEOR INTELLIGENTSIA) | Row 70 - Cell 2 |
| DNF | Nina Wollaston (ISCorp Pro Cycling) | Row 71 - Cell 2 |
| DNF | Kristen Arnold (THE METEOR INTELLIGENTSIA) | Row 72 - Cell 2 |
