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| 1 | Martina Sáblíková (Cze) Nowis Team | 0:37:41.547 |
| 2 | Martina Ružicková (Cze) Michela Fanini Record Rox | 0:01:54.174 |
| 3 | Jarmila Machacová (Cze) ASC Dukla Praha | 0:02:55.679 |
| 4 | Janka Števková (Svk) CK Epic Dohnany | 0:03:53.291 |
| 5 | Alžbeta Pavlendova (Svk) CK Dukla Bratislava | 0:04:27.111 |
| 6 | Monika Simonová (Cze) Cyklotrénink - František Trkal | 0:04:31.763 |
| 7 | Lenka Sýkorová (Cze) CK Kolokrám - Svijany | 0:04:36.698 |
| 8 | Kristína Lapinová (Svk) Bike Pro, S.R.O. | 0:04:48.155 |
| 9 | Monika Kadlecová (Svk) CK Epic Dohnany | 0:05:12.146 |
| 10 | Karolína Erbanová (Cze) Nowis Team | 0:05:57.968 |
| 11 | Klára Jandová (Cze) Comfort Plzen | 0:06:09.543 |
| 12 | Veronika Bláhová (Cze) Drásal Team Holešov | 0:06:27.016 |
| 13 | Katarína Hranaiová (Cze) AC Sperta Praha | 0:06:28.972 |
| 14 | Veronika Mrkvicová (Cze) TJ Favorit Brno | 0:06:38.257 |
| 15 | Tereza Trefná (Cze) Alltraining.Cz | 0:06:44.917 |
| 16 | Michaela Malariková (Svk) Ski-Bike Sportmed Bratislava | 0:07:13.882 |
| 17 | Romana Danecková (Cze) Mapie Cyklo Kankovský | 0:07:20.841 |
| 18 | Zuzana Vojtášová (Svk) CK Bánská Bystrica | 0:07:25.000 |
| 19 | Denisa Bartošová (Cze) SK Maxbike Orlová O.S. | 0:07:38.679 |
| 20 | Linda Vítová (Cze) TJ Cykloprag | 0:07:57.126 |
| 21 | Veronika Kotalová (Cze) ACK Stará Ves Nad Ondrejnicí | 0:08:02.201 |
| 22 | Jana Vycítalová (Cze) ACS Drak Vrbno | 0:09:04.357 |
| 23 | Jana Dubnová (Cze) Experiment 23 | 0:09:06.371 |
| 24 | Veronika Villímová (Svk) Cyklo-Team Habera Košice | 0:09:20.888 |
| 25 | Marcela Kovárová (Svk) Falange Bratislava | 0:09:55.292 |
| 26 | Karin Baumlová (Svk) Falange Bratislava | 0:10:08.354 |
| 27 | Júlia Jurcová (Svk) Falange Bratislava | 0:10:21.830 |
| 28 | Jana Cupová (Cze) CK Frenštát P. R. | 0:10:34.939 |
| 29 | Sylva Líbenková (Cze) Kolík Tj Bohumín | 0:10:35.972 |
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