| 1 | Chloe Woodruff (USA) Stan's No Tubes- Pivot | | |
| 2 | Georgia Gould (USA) Luna Pro Team | | |
| 3 | Katerina Nash (USA) Luna Pro Team | | |
| 4 | Erin Huck (USA) Scott 3 Roy | | |
| 5 | Eva Lechner (Ita) Luna Pro Team | | |
| 6 | Rebecca Beaumont (Can) Cyclone d'Alma/ Devinci | | |
| 7 | Lea Davison (USA) Specialized | | |
| 8 | Catharine Pendrel (Can) Luna Pro Team | | |
| 9 | Haley Smith (Can) Norco Factory Team | | |
| 10 | Evelyn Dong (USA) Cannondale 360 Fly | | |
| 11 | Rose Grant (USA) Stan's No Tubes- Pivot | | |
| 12 | Amanda Nauman (USA) SDG- Muscle Monster | | |
| 13 | Agustina Apaza (Arg) Argentina National Team | | |
| 14 | Malene Degn (Den) Danish National Team | | |
| 15 | Shayna Powless (USA) Liv Co-Factory | | |
| 16 | Hannah Finchamp (USA) Luna Pro Team | | |
| 17 | Maghalie Rochette (USA) Luna Pro Team | | |
| 18 | Jena Greaser (USA) Think Green-V02 Multisport p/b SWORD Cycling Team | | |
| 19 | Sofia Gomez Villafane (USA) Competitive Cyclist | - 2 laps | |
| 20 | Andre Waldiss (USA) Luna Pro Team | - 3 laps | |
| 21 | Suzanne Snyder (USA) Sierra Endurance Sports | | |
| 22 | Sammi Runnels (USA) Ridebiker p/b Sho-Air | | |
| 23 | Katie Button (Can) Procity Racing | | |
| 24 | Haley Batten (USA) Luna Pro Team | - 4 laps | |
| 25 | Serena Bishop Gordon (USA) Liv Co-Factory | - 5 laps | |
| 26 | Emma Maaranen (USA) Rolf Prima/ Trek | | |
| 27 | Alexis Skarda (USA) Stan's Notubes Elite Women's Team | | |
| 28 | Amy Beisel (USA) Team Ridebiker | | |
| 29 | Kayley Burdine (USA) Eastern Shore Cycles | | |
| 30 | Jennifer Malik (USA) Paradise Garage | | |
| 31 | Sarah Kaufmann (USA) Stan's Notubes Elite Women's Team | | |
| 32 | Emily Schaldach (USA) | | |
| 33 | Daniela Campuzano (Mex) Daniela Campuzano | | |
| 34 | Chloe Cross (Can) Team Whistler | | |
| 35 | Kristine Contento- (USA) MTBnj.Com | | |
| 36 | Luciana Roland (Arg) Argentina | | |
| 37 | Nina Baum (USA) Stan's Notubes Elite Women's Team | - 6 laps | |
| 38 | Rebecca Gross (USA) Khs | | |
| 39 | Rachel Pageau (Can) Devinci / Fox MTB | | |
| 40 | Elizabeth Gruber (USA) Team Lost Coast Brewery | | |
| 41 | Siobhan Kelly (Can) Epic Sports Performance | | |
| 42 | Abbey Grimmer (USA) Bike Like A Girl Elite Team | | |
| 43 | Jennifer Smith (USA) Stan's Notubes Elite Women's Team | | |
| 44 | Erica Tingey (USA) Jamis Cross Country Fact | | |
| 45 | Elizabeth Lurz (USA) Athletes On Track | | |
| 46 | Rebecca Blatt (USA) Van Diessel | | |
| 47 | Joy Adams (USA) Boise Velowmen | | |
| 48 | Kara Lapoint (USA) | | |
| 49 | Nora Richards (USA) | - 8 laps | |
| DNS | Kate Courtney (USA) Specialized | | |
| DNS | Ren Chengyuan (Chn) China Jiangsu ZGL MTB Team | | |
| DNS | Larissa Connors (USA) Team Ridebiker | | |
| DNS | Cindy Montambault (Can) | | |
| DNS | Mikaela Kofman (Can) | | |
| DNS | Frederique Trudel (Can) Specialized Canada | | |
| DNS | Catherine Fleury (Can) | | |
| DNS | Erin Alders (USA) | | |
| DNS | Karlee Gendron (Can) Trek Red Truck Racing | | |
| DNS | Jane Kerner (USA) Team Baghouse | | |
| DNS | Teal Stetson-Lee (USA) | | |
| DNS | Fairlee Frey (USA) | | |
| DNS | Laura Slavin (USA) Elite Endurance Pro Facto | | |
| DNS | Sophie Giovane (Fra) | | |