| 1 | Helen Wyman (GBr) Kona | 0:43:40 |
| 2 | Amanda Miller (USA) Team Optum p/b Kelly Benefit Strategies | 0:00:28 |
| 3 | Carmen McNellis Small (USA) Team Optum p/b Kelly Benefit Strategies | Row 2 - Cell 2 |
| 4 | Caroline Mani (Fra) Raleigh Clement | 0:00:57 |
| 5 | Jade Wilcoxson (USA) Team Optum p/b Kelly Benefit Strategies | 0:01:25 |
| 6 | Amanda Carey (USA) Volkswagen Boise | 0:01:37 |
| 7 | Maureen Bruno Roy (USA) Bob's Red Mill p/b Seven Cycles | Row 6 - Cell 2 |
| 8 | Courtenay McFadden (USA) Clif Bar/Bicycle Center | 0:01:42 |
| 9 | Jessica Cutler (USA) Cyclocrossracing.com | 0:03:08 |
| 10 | Rebecca Blatt (USA) Van Dessel Sports | 0:03:28 |
| 11 | Linda Sone (USA) Cycle-Smart Inc. | 0:03:36 |
| 12 | Abby Strigel (USA) Trek Cyclocross Collective | Row 11 - Cell 2 |
| 13 | Marne Smiley (USA) Bob's Red Mill Cyclocross Team | 0:04:38 |
| 14 | Corey Coogan-Cisek (USA) Cyclocrossracing.com | 0:04:59 |
| 15 | Rebecca Gross (USA) | 0:05:47 |
| 16 | Sierra Siebenlist (USA) Matthews Bicycle | Row 15 - Cell 2 |
| 17 | Amber Markey (USA) My Wife Inc | 0:06:00 |
| 18 | Elizabeth So (USA) Les Petites Victoires | 0:06:57 |
| 19 | Jeanne Fleck (USA) Velo Deluth | 0:07:02 |
| 20 | Robin Williams (USA) Iowa City Cycling Club / Mercy | 0:08:32 |
| 21 | Corrie Osborne (USA) Team Extreme | 0:08:59 |
| 22 | Karen Brocket (USA) Ethos Racing | Row 21 - Cell 2 |
