| 1 | Chloe Dygert (TWENTY16 Devo presented by SHO-) | 1:25:59 |
| 2 | Amy Phillips (UCI WPT: Pepper Palace Pro Cycl) | 0:00:54 |
| 3 | Daphne Karagianis (Chicago Cuttin Crew) | 0:01:54 |
| 4 | Holly Mathews (ISCorp Cycling pb SmartChoice M) | 0:01:55 |
| 5 | Danielle Smith (Tenspeed Hero) | 0:02:00 |
| 6 | Julie Kuliecza (UCI WPT: Pepper Palace Pro Cycl) | |
| 7 | Abby Krawczyk (ISCorp Cycling pb SmartChoice M) | |
| 8 | Jeannie Kuhajek (ISCorp Cycling pb SmartChoice M) | |
| 9 | Kelli Richter (PSIMET Racing) | |
| 10 | Jan Bennett (FCS|Cycling: pb Visit Dallas Cy) | |
| 11 | Jannette Rho (Chicago Cuttin Crew) | |
| 12 | Amanda Schaap (Speed Merchants) | |
| 13 | Sierra Siebenlist (Scarlet Fire) | |
| 14 | Sarah Szefi (PSIMET Racing) | |
| 15 | Ellen Ryan (xXx Racing) | |
| 16 | Carol Jeane Sansome (SPBRC-Grand Performance) | |
| 17 | Kathryne Carr | |
| DNF | Emily Bramel (SPBRC-Grand Performance) | |
| DNF | Sarah Demerly (First Internet Bank Cycling Team) | |
