| 1 | Katerina Nash (Cze) Luna Pro Team | 0:37:29 |
| 2 | Nicole Duke (USA) Marin-Spy | 0:00:10 |
| 3 | Rachel Lloyd (USA) California Giant Strawberries-Specialized | 0:00:11 |
| 4 | Caroline Mani (Fra) Raleigh-Clement | 0:00:40 |
| 5 | Amanda Nauman (USA) SDG-Felt | 0:00:53 |
| 6 | Beth Ann Orton (USA) Team S&M | 0:00:59 |
| 7 | Emily Kachorek (USA) #Ghetomoto | |
| 8 | Serena Gordon (USA) All Access Racing | 0:01:17 |
| 9 | Carolina Gomez-Villafane (Arg) Team Rambuski Law | 0:01:58 |
| 10 | Hannah Finchamp (USA) Team Luna Chix | 0:02:22 |
| 11 | Alice Pennington (USA) Team S&M-Kona | 0:02:28 |
| 12 | Rebecca Blatt (USA) Van Dessel | 0:03:11 |
| 13 | Elicia Hildebrand (USA) Trek Cyclocross Collective | 0:03:42 |
| 14 | Anissa Cobb (USA) | 0:05:04 |
| 15 | Maddie Melcher (USA) The TEAM SoCalCross | 0:05:30 |
