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| Date | April 18, 2025 |
| Start location | Lennik, Belgium |
| Finish location | Overijse, Belgium |
| Length | 125.7km |
| UCI class | 1.Pro |
| Last edition | 2024 De Brabantse Pijl Women |
| Previous winner | Elisa Longo Borghini |
2025 Brabantse Pijl Dames route
2025 Brabantse Pijl Dames climbs
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| Climb | Length | Max Grade | Avg Grade | Km raced | Km to go |
|---|---|---|---|---|---|
| Beerselberg | 500 m | 10.10% | 7.90% | 12.2 | 113.5 |
| Bruineput | 1200 m | 10.90% | 5.70% | 18.3 | 107.4 |
| Eigenbrakelsesteenweg | 900 m | 6.10% | 4.90% | 24 | 101.7 |
| Chemin dit le Bois | 1400 m | 5.70% | 3.60% | 30.4 | 95.3 |
| Rue François Dubois | 900 m | 5.90% | 4.10% | 47.2 | 78.5 |
| Holstheide | 1000 m | 11.50% | 5.30% | 59 | 66.7 |
| S-Bocht Overijse | 1300 m | 5.70% | 4.20% | 66.1 | 59.6 |
| Hertstraat | 700 m | 9.40% | 4.70% | 70.7 | 55 |
| Moskesstraat | 500 m | 9.20% | 14.90% | 75.8 | 49.9 |
| Holstheide | 1000 m | 11.50% | 5.30% | 78.8 | 46.9 |
| S-Bocht Overijse | 1300 m | 5.70% | 4.20% | 85.9 | 39.8 |
| Hertstraat | 700 m | 9.40% | 4.70% | 90.4 | 35.3 |
| Moskesstraat | 500 m | 9.20% | 14.90% | 95.6 | 30.1 |
| Holstheide | 1000 m | 11.50% | 5.30% | 98.6 | 27.1 |
| S-Bocht Overijse | 1300 m | 5.70% | 4.20% | 105.7 | 20 |
| Hertstraat | 700 m | 9.40% | 4.70% | 110.3 | 15.4 |
| Moskesstraat | 500 m | 9.20% | 14.90% | 115.4 | 10.3 |
| Holstheide | 1000 m | 11.50% | 5.30% | 118.4 | 7.3 |
| S-Bocht Overijse | 1300 m | 5.70% | 4.20% | 125.5 | 0.2 |
Brabantse Pijl Dames start list
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