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| 1 | Radomir Simunek (Cze) BKCP - Powerplus | 1:00:00 |
| 2 | Martin Bina (Cze) CEZ Cyklo Team Tábor | Row 1 - Cell 2 |
| 3 | Lubomir Petrus (Cze) BKCP - Powerplus | 0:00:12 |
| 4 | Jakub Skála (Cze) CEZ Cyklo Team Tábor | 0:00:44 |
| 5 | Petr Dlask (Cze) Madeta - Fitness / Specialized | 0:00:48 |
| 6 | Jan Nesvadba (Cze) Madeta - Fitness / Specialized | 0:00:58 |
| 7 | Tomáš Paprstka (Cze) Remerx-Merida Team Kolín | 0:00:59 |
| 8 | Jirí Polnický (Cze) Whirlpool - Author | 0:01:11 |
| 9 | David Kášek (Cze) CEZ Cyklo Team Tábor | 0:01:28 |
| 10 | Vladimír Kyzivát (Cze) Johnson Controls TJ Auto Škoda | 0:01:38 |
| 11 | Robert Gavenda (Svk) Dukla Trencín Trek | 0:01:42 |
| 12 | Michael Boroš (Cze) CEZ Cyklo Team Tábor | 0:01:44 |
| 13 | Ondrej Bambula (Cze) CEZ Cyklo Team Tábor | 0:02:22 |
| 14 | Radek Polnický (Cze) Madeta - Fitness / Specialized | 0:02:25 |
| 15 | Filip Eberl (Cze) Remerx-Merida Team Kolín | Row 14 - Cell 2 |
| 16 | Martin Haring (Svk) CK Banská Bystrica | 0:02:56 |
| 17 | Vaclav Metlicka (Svk) Johnson Controls TJ Auto Škoda | 0:03:17 |
| 18 | Filip Adel (Cze) KC Hlinsko | 0:03:58 |
| 19 | Matej Lasák (Cze) Max Cursor | 0:04:19 |
| 20 | Tomáš Podrazil (Cze) TJ Sokol Holé Vrchy | 0:04:33 |
| 21 | Tomáš Bohata (Cze) Remerx-Merida Team Kolín | 0:05:06 |
| 22 | Vojtech Nipl (Cze) Focus Cycling Znojmo | 0:05:14 |
| 23 | Ondrej Glajza (Svk) Trek KCK Oslany | 0:05:55 |
| 24 | Bretislav Rohel (Cze) Bikestyle.cz | Row 23 - Cell 2 |
| 25 | Stanislav Bambula (Cze) CEZ Cyklo Team Tábor | Row 24 - Cell 2 |
| 26 | Petr Hampl (Cze) Tj Favorit Brno | Row 25 - Cell 2 |
| 27 | Jan Svoboda (Cze) Yogi Racing Ostrava | Row 26 - Cell 2 |
| 28 | Michal Benda (Cze) TJ Sokol Plavy-Cyklo Benda | Row 27 - Cell 2 |
| 29 | Grzegorz Grabarek (Pol) Niestowarzszony | Row 28 - Cell 2 |
| 30 | Tomáš Janošek (Cze) ACK Stará Ves n. Ondrejnicí | Row 29 - Cell 2 |
| 31 | Tomáš Novácek (Cze) S.K. Jirí Team Ostrava | Row 30 - Cell 2 |
| 32 | Jakub Rydval (Cze) KC Kooperativa SG Jablonec N.N | Row 31 - Cell 2 |
| 33 | Jirí Rehák (Cze) Johnson Controls TJ Auto Škoda | Row 32 - Cell 2 |
| 34 | Tomáš Jakl (Cze) S.K. Jirí Team Ostrava | Row 33 - Cell 2 |
| 35 | Lukáš Venc (Cze) Mapei Cyklo Kankovský | Row 34 - Cell 2 |
| 36 | Vít Rehák (Cze) Remerx-Merida Team Kolín | Row 35 - Cell 2 |
| 37 | Lukáš Mitas (Cze) RBB Invest Jindrichuv Hradec | Row 36 - Cell 2 |
| 38 | Zbynek Holubovský (Cze) Galaxy Cyklošvec | Row 37 - Cell 2 |
| 39 | Michal Bednár (Cze) CEZ Cyklo Team Tábor | Row 38 - Cell 2 |
| 40 | Marek Sedlácek (Cze) S.K. Jirí Team Ostrava | Row 39 - Cell 2 |
| 41 | Tomáš Stolarík (Cze) S.K. Jirí Team Ostrava | Row 40 - Cell 2 |
| 42 | Tomáš Holický (Cze) RBB Invest Jindrichuv Hradec | Row 41 - Cell 2 |
| 43 | Tomáš Nový (Cze) CEZ Cyklo Team Tábor | Row 42 - Cell 2 |
| 44 | Tomáš Havelka (Cze) TJ Lokomotiva Beroun | Row 43 - Cell 2 |
| 45 | Václav Svatoš (Cze) TJ Sokol Holé Vrchy | Row 44 - Cell 2 |
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