The Dutchwoman, who is among the favourites for Wednesday's time trial, was riding with teammate Annemiek Van Vleuten when the incident occurred.
She was stopped at the circuit and was reportedly sent off balance by the officials, according to a report by WielerFlits.
"Because there were some women on the track who rode around unchecked, the security was very strict," Van der Breggen told WielerFlits. "We were checked a first time and when we passed that point again, we wanted to continue but the guards jumped in front of our bikes.
"Not much more happened. In any case, the incident has no consequences for my Olympic time trial tomorrow. I want to focus on that fully now."
A video posted to Twitter by Japanese cycling coach Kyosuke Takei showed several riders waiting outside the circuit, with Van der Breggen off her bike talking to officials.
"Everyone had to stop because of an error by the organisers," Japanese cycling coach Kyosuke Takei told Wielerflits. "In the turmoil with the guards, Anna was threatened and pulled down.
"It was a big mess today at the final training of the time trial. It was sad what happened. The organisers have no respect for the riders. I understand that everyone is trying to do their best in their position, but this was very confusing. I haven't seen anything like that in a long time."
Van der Breggen, who is the reigning world time trial champion and took bronze in the discipline in the 2016 Games in Rio, wasn't injured in the incident and will be able to race the time trial as normal.
The incident is the latest to befall Dutch riders at the Tokyo Games. At Monday's men's mountain bike race, Mathieu van der Poel crashed out of contention after misjudging a jump when he thought there would be a wooden ramp available. On the same day, BMX rider Niek Kimmann suffered a knee injury while training after colliding with an official who suddenly tried to cross the track.
Meanwhile, at the women's road race, a mix-up in communication in the Dutch team saw Van Vleuten mistakenly celebrate victory as she crossed the finish line, unaware that gold medallist Anna Kiesenhofer had already finished, winning from the early breakaway.
😢🇯🇵😢🇯🇵😢@raykerckhoffs 👋… So sorry big problem…#Tokyo2020 pic.twitter.com/zYFpu6QjicJuly 27, 2021
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