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| 1 | Martin Bína (Cze) Cyklo Team Budvar Tábor | 1:02:52 |
| 2 | Zdenek Mlynár (Cze) Max Cursor | 0:00:07 |
| 3 | Martin Zlámalík (Cze) Volvo Auto Hase Mtb Team | Row 2 - Cell 2 |
| 4 | Petr Dlask (Cze) Telenet Fidea Cycling Team | 0:00:10 |
| 5 | Robert Gavenda (Svk) Telenet Fidea Cycling Team | Row 4 - Cell 2 |
| 6 | Lukáš Kloucek (Cze) Sunweb - Projob Cycling Team | Row 5 - Cell 2 |
| 7 | Ondrej Bambula (Cze) Cyklo Team Budvar Tábor | 0:00:37 |
| 8 | David Kášek (Cze) Cyklo Team Budvar Tábor | 0:00:42 |
| 9 | Kamil Ausbuher (Cze) Prodoli Remerx Team | 0:00:45 |
| 10 | Jirí Polnický (Cze) Sunweb - Projob Cycling Team | Row 9 - Cell 2 |
| 11 | Vladimír Kyzivát (Cze) Johnson Controls Aš Mb | 0:02:07 |
| 12 | Johannes Sickmüller (Ger) Harvestehuder Rsv V. 1909 | 0:02:31 |
| 13 | David Menger (Cze) Prodoli Remerx Team | 0:02:53 |
| 14 | Lukáš Príhoda (Cze) Klub Cyklistiky Hlinsko | 0:03:05 |
| 15 | Robert Glajza (Svk) Bkp | Row 14 - Cell 2 |
| 16 | Filip Adel (Cze) Cyklo Team Budvar Tábor | 0:03:28 |
| 17 | Ole Quast (Ger) Harvestehuder Rsv V. 1909 | 0:03:35 |
| 18 | Stanislav Bambula (Cze) Cyklo Team Budvar Tábor | 0:03:41 |
| 19 | Martin Haring (Svk) Ck Banská Bystrica | 0:03:51 |
| 20 | Karel Nepraš (Cze) Ack Stará Ves N. Ondrejnicí | 0:04:05 |
| 21 | Petr Novotný (Cze) Tj Stadion Louny | 0:04:27 |
| 22 | Christoph Pfingsten (Ger) Berliner Tsc | 0:04:45 |
| 23 | Tomáš Podrazil (Cze) Tj Sokol Holé Vrchy | 0:05:52 |
| 24 | Jan Chrobák (Cze) Johnson Controls Aš Mb | 0:06:40 |
| -1 lap | Bretislav Rohel (Cze) Forman Cycling Team | Row 24 - Cell 2 |
| -1 lap | Michal Benda (Cze) Johnson Controls Aš Mb | Row 25 - Cell 2 |
| -1 lap | Michael Cerný (Cze) Cyklo Team Budvar Tábor | Row 26 - Cell 2 |
| -1 lap | Martin Rehák (Cze) Scott Scania Team Kolín | Row 27 - Cell 2 |
| -1 lap | Martin Hunal (Cze) Velosport Valenta Scott Team | Row 28 - Cell 2 |
| -2 laps | Jaroslav Musil (Cze) Cyklo Team Budvar Tábor | Row 29 - Cell 2 |
| -2 laps | Jirí Brázdil (Cze) Johnson Controls Aš Mb | Row 30 - Cell 2 |
| -2 laps | Heinrich Berger (Ger) Harvestehuder Rsv V. 1909 | Row 31 - Cell 2 |
| -5 laps | Dries Pauwels (Bel) Empella Czech Team | Row 32 - Cell 2 |
| -5 laps | Jakub Rohlík (Cze) Cyklo Team Budvar Tábor | Row 33 - Cell 2 |
| -5 laps | Lukáš Kovanda (Cze) Cyklo Team Budvar Tábor | Row 34 - Cell 2 |
| -5 laps | Stefan Freudenberg (Ger) Dresdner Sc 1898 | Row 35 - Cell 2 |
| -6 laps | Martin Frano (Svk) Ck Banská Bystrica | Row 36 - Cell 2 |
| -6 laps | Tomáš Nový (Cze) Cyklo Team Budvar Tábor | Row 37 - Cell 2 |
| -6 laps | Ladislav Kopec (Cze) Tj Lokomotiva Beroun | Row 38 - Cell 2 |
| -6 laps | Ondrej Pipek (Cze) Scott Scania Team Kolín | Row 39 - Cell 2 |
| -7 laps | Jakub Holas (Cze) Tj Sokol Holé Vrchy | Row 40 - Cell 2 |
| -7 laps | Milan Svoboda (Cze) Scott Scania Team Kolín | Row 41 - Cell 2 |
| -8 laps | Josef Suchý (Cze) Johnson Controls Aš Mb | Row 42 - Cell 2 |
| -8 laps | Lukáš Poríz (Cze) Ct Mladá Boleslav | Row 43 - Cell 2 |
| -9 laps | Tomáš Havelka (Cze) Tj Lokomotiva Beroun | Row 44 - Cell 2 |
| -9 laps | Tomáš Ženíšek (Cze) Tj Zce Cyklistika Plzen | Row 45 - Cell 2 |
| -9 laps | Jirí Sotona (Cze) Ct Mladá Boleslav | Row 46 - Cell 2 |
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