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| 1 | Jaroslav Kulhavý (Cze) Rubena-Specialized Cycling Team | 1:00:03 |
| 2 | Ondrej Bambula (Cze) Cyklo Team Budvar Tábor | 0:00:02 |
| 3 | Kamil Ausbuher (Cze) Prodoli Remerx Team | Row 2 - Cell 2 |
| 4 | Vladimír Kyzivát (Cze) JOHNSON CONTROLS AŠ MB | 0:00:05 |
| 5 | Jan Škarnitzl (Cze) DIMP - GIANT team | 0:00:06 |
| 6 | Petr Novotný (Cze) TJ Stadion Louny | 0:01:06 |
| 7 | Václav Metlicka (Svk) Prodoli Remerx Team | 0:01:28 |
| 8 | David Kášek (Cze) Cyklo Team Budvar Tábor | 0:01:29 |
| 9 | David Menger (Cze) Prodoli Remerx Team | 0:01:56 |
| 10 | Kristián Hynek (Cze) Scott&Hagget MTB | 0:02:13 |
| 11 | Josef Soukup (Cze) CK KOLOKRÁM.CZ - SVIJANY | 0:02:37 |
| 12 | Filip Adel (Cze) Cyklo Team Budvar Tábor | 0:02:49 |
| 13 | Robert Glajza (Svk) BKP | 0:02:55 |
| 14 | Filip Eberl (Cze) SCOTT SCANIA TEAM Kolín | 0:03:01 |
| 15 | Michal Vlcek (Cze) KC KOOPERATIVA SG JABLONEC n.N | 0:03:10 |
| 16 | Karel Nepraš (Cze) ACK Stará Ves n. Ondr(ejnicí | 0:03:58 |
| 17 | Stanislav Bambula (Cze) Cyklo Team Budvar Tábor | 0:04:05 |
| 18 | Bretislav Rohel (Cze) FORMAN Cycling Team | 0:04:39 |
| 19 | Tomáš Janošek (Cze) ACK Stará Ves n. Ondr(ejnicí | 0:05:50 |
| 20 | Jakub Friml (Cze) SCOTT SCANIA TEAM Kolín | Row 19 - Cell 2 |
| 21 | Jirí Hudecek (Cze) Scott&Hagget MTB | 0:06:12 |
| 22 | Jirí Podrazil (Cze) TJ Sokol Holé Vrchy | 0:06:21 |
| 23 | Jan Klabouch (Cze) Cyklo Team Budvar Tábor | 0:06:40 |
| 24 | Martin Haring (Svk) CK Banská Bystrica | 0:06:59 |
| 25 | Jakub Rohlík (Cze) Cyklo Team Budvar Tábor | 0:07:20 |
| 26 | Tomáš Podrazil (Cze) TJ Sokol Holé Vrchy | 0:07:47 |
| 27 | Michal Benda (Cze) JOHNSON CONTROLS AŠ MB | 0:08:43 |
| 28 | Martin Rehák (Cze) SCOTT SCANIA TEAM Kolín | Row 27 - Cell 2 |
| 29 | Josef Suchý (Cze) JOHNSON CONTROLS AŠ MB | Row 28 - Cell 2 |
| 30 | Tomáš Nový (Cze) Cyklo Team Budvar Tábor | Row 29 - Cell 2 |
| 31 | Ondrej Pipek (Cze) SCOTT SCANIA TEAM Kolín | Row 30 - Cell 2 |
| 32 | Petr Linhart (Cze) TJ Sokol Holé Vrchy | Row 31 - Cell 2 |
| 33 | Marek Farkaš (Cze) TJ Lokomotiva Beroun | Row 32 - Cell 2 |
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