| 1 | Clara Hughes (Can) Canadian National Team | 0:23:49 | |
| 2 | Hanna Solovey (Ukr) Ukrainian National Team | 0:00:10 | |
| 3 | Amber Neben (USA) HTC Highroad Women | 0:00:13 | |
| 4 | Jeannie Longo-Ciprelli (Fra) French National Team | 0:00:50 | |
| 5 | Julie Beveridge (Can) Vienne Futuroscope | 0:01:11 | |
| 6 | Sue Schlatter (Can) Stevens Pro Team | 0:01:15 | |
| 7 | Denise Ramsden (Can) Juvederm - Specialized | 0:01:25 | |
| 8 | Heather Logan (Can) Colavita Forno d'Asolo | 0:01:30 | |
| 9 | Lesya Kalitovska (Ukr) Ukrainian National Team | 0:01:35 | |
| 10 | Edwige Pitel (Fra) Vienne Futuroscope | 0:01:39 | |
| 11 | Megan Guarnier (USA) TIBCO - To The Top | 0:01:41 | |
| 12 | Svitlana Galyuk (Ukr) Ukrainian National Team | | |
| 13 | Anna Harkowska (Pol) Kallisto | 0:01:44 | |
| 14 | Rushlee Buchanan (NZl) Colavita Forno d'Asolo | 0:01:45 | |
| 15 | Caroline Ryan (Irl) Irish National Team | 0:01:49 | |
| 16 | Veronique Labonte (Can) Juvederm - Specialized | 0:01:53 | |
| 17 | Erinne Willock (Can) TIBCO - To The Top | | |
| 18 | Cynthia Nelson (Can) Reform-Body/Power | 0:02:02 | |
| 19 | Leah Kirchmann (Can) Manitoba | 0:02:13 | |
| 20 | Annick Dufour (Can) Stevens Pro Team | 0:02:21 | |
| 21 | Audrey Cordon (Fra) Vienne Futuroscope | 0:02:31 | |
| 22 | Stephanie Skoreyko (Can) Kallisto | 0:02:34 | |
| 23 | Véronique Fortin (Can) Equipe Quebec | 0:02:35 | |
| 24 | Olivia Dillon (Irl) Irish National Team | 0:02:36 | |
| 25 | Moriah Macgregor (Can) Juvederm - Specialized | 0:02:45 | |
| 26 | Jennifer Stephenson (Can) Reform-Body/Power | 0:02:57 | |
| 27 | Tricia Spooner (Can) Stevens Pro Team | 0:03:02 | |
| 28 | Annie Ewart (Can) Canadian National Team | | |
| 29 | Marti Shea (USA) Destination Cycling | 0:03:03 | |
| 30 | Sue Palmer-Komar (Can) P-K Express/HNZ St | 0:03:11 | |
| 31 | Audrey Sarrazin (Can) Equipe Quebec | 0:03:24 | |
| 32 | Adriane Provost (Can) Equipe Quebec | 0:03:45 | |
| 33 | Julia Garnet (Can) Canadian National Team | 0:04:00 | |
| 34 | Allison Lampi (Can) Reform-Body/Power | 0:04:07 | |
| 35 | Suzie Brown (Can) P-K Express/HNZ St | 0:04:28 | |