| 1 | Natasha Elliott (Can) Garneau Club Chaussure-Ogilvy | 0:41:28 |
| 2 | Rebecca Blatt (USA) Team Kenda | 0:01:34 |
| 3 | Nikki Thiemann (USA) Team CF | 0:01:36 |
| 4 | Melissa Bunn (Can) Stevens Racing p/b The Cyclery | 0:01:49 |
| 5 | Arley Kemmerer (USA) C3 - Athletes Serving Athletes | 0:02:48 |
| 6 | Carolyn Popovic (USA) PAValleys.com | 0:02:50 |
| 7 | Sarah Krzysiak (USA) NYCross.com / CBRC | 0:03:17 |
| 8 | Sophie Matte (Can) Stevens Racing p/b The Cyclery | 0:03:43 |
| 9 | Kristine Church (USA) Human Zoom-Pabst Blue Ribbon | 0:04:25 |
| 10 | Natalie Stoll (USA) MVP Health Care Cycling | 0:04:50 |
| 11 | Kristin Gavin (USA) Team CF | 0:05:05 |
| 12 | Leslie Chown Honsberger (Can) Team CF | 0:08:49 |
| 13 | Beth Mason (USA) Verge Test Pilot | |
