| 1 | Lauretta Hanson (Fearless Femme p/b Haute Wheels) | 1:00:14 | |
| 2 | Laura Van Gilder (Mellow Mushroom Racing) | | |
| 3 | Colleen Hayduk (Argon18Racing p/b November &Fas) | | |
| 4 | Lauren Dagostino (Healthy and Happy Racing) | | |
| 5 | Lenore Pipes (Van Dessel Factory Team) | | |
| 6 | Kristin Lotito (Houlihan Lokey) | | |
| 7 | Rebecca Chan (ABRT (Annapolis Bicycle Racing) | | |
| 8 | Monica Volk (Team EPS/CSS p/b Shebell &Shebe) | | |
| 9 | Laura Summers (Greater Hartford Cycling Club) | | |
| 10 | Colleen Gulick (Team EPS/CSS p/b Shebell &Shebe) | | |
| 11 | Mary Costelloe (Alliance Environmental) | | |
| 12 | Meredith Uhl (Farm Team Elite Women's Cycling) | | |
| 13 | Emily Spence (Healthy and Happy Racing) | | |
| 14 | Vanessa Drigo (Argon18Racing p/b November &Fas) | | |
| 15 | Kristie Nichols James (QCW Breakawaybikes.com p.b. Fel) | | |
| 16 | Meredith Ehn (CAWES p/b Specialized) | | |
| 17 | Emily Underwood (Zimmer Capital Orthopedics p/b) | | |
| 18 | Camie Kornely (Breakaway/NSX/Carve Systems) | | |
| 19 | Jessica Chong | | |
| 20 | Patricia Buerkle (The Weather Channel Giant Cycli) | | |
| 21 | Elspeth Huyett (South Mountain Cycle & Cafe) | | |
| 22 | Masha Schneider | | |
| 23 | Sue McQuiston (Annapolis Bicycle Racing Team) | | |
| 24 | Arley Kemmerer (Mid-Atlantic Colavita Women's T) | | |
| 25 | Hannah Todd (Stan's NoTubes p/b Velo Classic) | 0:00:04 | |
| 26 | CarolLynn Mills (Bicycle Roots p/b Hatchmap) | | |
| 27 | Marianna Williams (QCW Breakawaybikes.com p.b. Fel) | 0:00:14 | |
| 28 | Dana Stryk (Evolution Cycling Club p/b Long) | | |
| 29 | Renee Engelhardt (Radical Media) | | |
| 30 | Jacqueline Paull (Team ProPower Coaching) | | |
| 31 | Fiona Meade (Fearless Femme p/bHaute Wheels) | 0:00:18 | |
| 32 | Tiffany Pezzulo (DNA Cycling p/b K4) | 0:00:22 | |
| 33 | Tess Senty (QCW Breakawaybikes.com p.b. Fel) | 0:00:23 | |
| 34 | Sara Ferrara (Penn State) | 0:00:27 | |
| 35 | Lenore Imhof (Team Montclair Bikery) | 0:00:46 | |
| 36 | Kacey Lloyd (Fearless Femme p/b Haute Wheels) | 0:00:55 | |
