| 1 | Laura Van Gilder (USA) Van Dessel p/b Mellow Mushroom | 0:45:42 |
| 2 | Arley Kemmerer (USA) C3-Twenty20 Cycling | 0:00:01 |
| 3 | Nicole Thiemann (USA) Team CF | 0:00:04 |
| 4 | Cassandra Maximenko (USA) Rare Vos Racing | Row 3 - Cell 2 |
| 5 | Stacey Barbossa (USA) NJ/NY Colavita Women | 0:01:15 |
| 6 | Christina Birch (USA) JAM Fund-NCC | 0:01:30 |
| 7 | Brittlee Bowman (USA) House Industries-Richard Sachs-RGM | 0:02:02 |
| 8 | Katrina Dowidchuk (USA) Colavita-EspnW NJ | 0:02:19 |
| 9 | Kathryn Cumming (USA) Team Yacht Club | 0:02:35 |
| 10 | Allyson Tufano (USA) Sportif Coaching Group-Patapsco Bike | 0:03:02 |
| 11 | Joanne Grogan (USA) Cyclocrossracing.com | 0:03:17 |
| 12 | Carolyn Popovic (USA) Team CF | 0:03:26 |
| 13 | Lauren Dagostino (USA) NJ/NY Colavita Women | 0:03:35 |
| 14 | Tara Parsons (USA) | 0:03:36 |
| 15 | Erin Silliman (USA) | 0:03:38 |
| 16 | Alice Henriques (USA) | 0:03:57 |
| 17 | Victoria Gates (USA) JAM Fund-NCC | 0:04:01 |
| 18 | Erica Yozell (USA) Bicycle Therapy-Melitta | 0:04:52 |
| 19 | Erin Faccone (USA) | 0:05:11 |
| 20 | Erin Mascelli (USA) Peanut Butter & Co. Human Zoom | 0:05:27 |
| 21 | Kristine Church (USA) Peanut Butter & Co. Human Zoom | 0:05:35 |
| 22 | Bailey Semian (USA) C3-Twenty20 Cycling | 0:05:40 |
| 23 | Elizabeth Bonilla (USA) | 0:06:15 |
| 24 | Stephanie Thompson (USA) WD Racing -NYC Velo | 0:07:01 |
| 25 | Shane Ferro (USA) | 0:07:28 |
| 26 | Heidi Wood (USA) Alki-Rubicon | 0:07:46 |
| 27 | Jessica Kutz (USA) Team EPS-CSS p/b Shebell & Shebell | Row 26 - Cell 2 |
| 28 | Lauren Festa (USA) Peanut Butter & Co. Human Zoom | Row 27 - Cell 2 |
