| 1 | Katerina Nash (Cze) Luna Pro Team | 0:37:35 | |
| 2 | Rachel Lloyd (USA) | 0:00:06 | |
| 3 | Nicole Duke (USA) Marin-Spy | 0:00:07 | |
| 4 | Courtenay McFadden (USA) Bicycle Blue Book-HRS-Rock Lobster | 0:00:21 | |
| 5 | Caroline Mani (Fra) Raleigh-Clement | 0:00:49 | |
| 6 | Amanda Nauman (USA) SDG-Felt | 0:00:57 | |
| 7 | Emily Kachorek (USA) #Ghetomoto | 0:01:05 | |
| 8 | Serena Gordon (USA) All Access Racing | 0:01:05 | |
| 9 | Beth Ann Orton (USA) Team S&M | 0:01:29 | |
| 10 | Rebecca Blatt (USA) Van Dessel | 0:01:30 | |
| 11 | Carolina Gomez-Villafane (Arg) Team Rambuski Law | 0:01:31 | |
| 12 | Sofia Gomez Villafane (Arg) Fort Lewis College | 0:02:55 | |
| 13 | Hannah Rae Finchamp (USA) | 0:03:15 | |
| 14 | Elicia Hildebrand (USA) | 0:03:36 | |
| 15 | Madelaine Melcher (USA) | 0:05:15 | |
| 16 | Anissa Cobb (USA) | 0:05:53 | |
| 17 | Stace Cooper (USA) TBBCX-Gheto | 0:07:46 | |
| DNF | Alice Pennington (USA) | | |
| DNS | Katie Jay Melena (USA) Bicycle Blue Book-HRS-Rock Lobster | | |