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The 2016 edition of Tirreno-Adriatico gets underway on Wednesday with a flat, fast 22.7km team time trial on the Lido di Camaiore, and Alejandro Valverde's Movistar squad will be the first team down the ramp at 1:45 local time, followed five minutes later by the Tinkoff Team of world champion Peter Sagan.
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| 1 | Movistar Team | 13:45:00 |
| 2 | Tinkoff Team | 13:50:00 |
| 3 | Dimension Data | 13:55:00 |
| 4 | Team LottoNl-Jumbo | 14:00:00 |
| 5 | CCC Sprandi Polkowice | 14:05:00 |
| 6 | FDJ | 14:10:00 |
| 7 | Trek-Segafredo | 14:15:00 |
| 8 | Cannondale Pro Cycling | 14:20:00 |
| 9 | Orica-GreenEdge | 14:25:00 |
| 10 | Team Katusha | 14:30:00 |
| 11 | Caja Rural-Seguros RGA | 14:35:00 |
| 12 | IAM Cycling | 14:40:00 |
| 13 | Bardiani CSF | 14:45:00 |
| 14 | Etixx - Quick-Step | 14:50:00 |
| 15 | Team Giant-Alpecin | 14:55:00 |
| 16 | Bora-Argon 18 | 15:00:00 |
| 17 | Team Sky | 15:05:00 |
| 18 | BMC Racing Team | 15:10:00 |
| 19 | Lotto Soudal | 15:15:00 |
| 20 | Lampre - Merida | 15:20:00 |
| 21 | AG2R La Mondiale | 15:25:00 |
| 22 | Androni Giocattoli - Sidermec | 15:30:00 |
| 23 | Astana Pro Team | 15:35:00 |
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